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当前位置: 首页 > 产品中心 > Polyclonal_antibody > Clontech/Cre重组酶Gesicles/200 uL/631449
商品详细Clontech/Cre重组酶Gesicles/200 uL/631449
Clontech/Cre重组酶Gesicles/200 uL/631449
Clontech/Cre重组酶Gesicles/200 uL/631449
商品编号: 631449
品牌: Takara
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多克隆抗体
公司分类: Polyclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Deliver Cre recombinase protein to mammalian cells in the absence of nucleic acids (i.e., no plasmid or virus is required), rapidly and efficiently. These gesicles are cell-derived nanovesicles containing concentrated Cre recombinase protein that, when applied to your target cells, fuse with the plasma membrane and discharge into the lumen of the cell.

Deliver Cre recombinase protein to mammalian cells in the absence of nucleic acids (i.e., no plasmid or virus is required), rapidly and efficiently. These gesicles are cell-derived nanovesicles containing concentrated Cre recombinase protein that, when applied to your target cells, fuse with the plasma membrane and discharge into the lumen of the cell. The enzyme is transported to the nucleus via its nuclear localization signal where it can carry out Cre-mediated recombination between loxP sites. Since there is no coding gene present, there is no risk of sustained Cre recombinase expression in your target cells, thus limiting the chance of unintended recombination events.

What is Cre recombinase?

Cre recombinase is a site-specific recombinase isolated from P1 bacteriophage that catalyzes the recombination between two 34­-bp loxP sites. The enzyme is widely used for knock-out and knock-in studies: any DNA sequence located between two tandem repeats of loxP can be excised to create a deletion mutant, or a plasmid containing a single loxP site can undergo site-specific integration into a genome containing loxP to create an insertion mutant.

What are the benefits of gesicle technology?

  • Far simpler to use than either plasmid or viral gene delivery. Simply apply 10–20 µl of the gesicles to your target cells; no preparation or pre-treatment is required.
  • Flox your cells on demand: Cre recombinase is present when you need it and gone when you don’t.
  • Can be used with many cell types, including dividing and non-dividing cells, primary cells, and cell lines.
  • Ensure efficient delivery and high activity of Cre recombinase (expand the Details tab in the product table to see data images). When using gesicle delivery, there is no delay for transcription/translation: the protein is present and active as soon as you apply the gesicles to your cells.
  • Since there is no coding gene present, there is no risk of sustained Cre recombinase expression in your target cells. It is present when you need it and gone when you don’t, so you avoid unwanted recombination events.
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品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。