Our Guide-it in vitro transcription and screening kits for sgRNA include four kits for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies. Each kit provides enough materials for 50 reactions. TheGuide-it sgRNAIn VitroTranscription Kit(Cat. # 632635) is used to produce high yields of sgRNAs viain vitrotranscription.
Our Guide-it in vitro transcription and screening kits for sgRNA include four kits for the simple production, cleanup, and evaluation of single guide RNAs (sgRNAs) for CRISPR/Cas9 studies. Each kit provides enough materials for 50 reactions. TheGuide-it sgRNAIn VitroTranscription Kit(Cat. # 632635) is used to produce high yields of sgRNAs viain vitrotranscription. This kit also includes the Guide-it IVT RNA Clean-Up Kit (Cat. # 632638), which is used to quickly purify sgRNA following in vitro transcription (IVT) reactions. Purified sgRNA from this kit is ready for direct use in transfection, electroporation, or in vitro cleavage assays. TheGuide-it sgRNA Screening Kit (Cat. # 632639)enables you to test the efficacy of different single guide RNAs (sgRNAs)in vitroprior to using them in studies involving Cas9-mediated gene editing so that you don"t waste time delivering ineffective sgRNAs to your cells. With this kit, a template containing a sgRNA target site is created by PCR, then combined with a sgRNA that you wish to test and recombinant Cas9 nuclease. The efficiency with which Cas9 nuclease cleaves the template can be measured using agarose gel electrophoresis. TheGuide-it Complete sgRNA Screening System (Cat. # 632636) includes all of the kit components described above and is used to synthesize and test the efficacy of single guide RNAs (sgRNAs). The kit contains all the reagents needed toin vitro transcribe and purify sgRNAs, and test their efficacy against a PCR target fragment using recombinant Cas9 nuclease.
