TB Green Premix Ex Taq II (Tli RNaseH Plus) is a TB Green Master Mix that effectively suppresses amplification of primer dimers and off-target products, and is therefore recommended for real-time PCR (qPCR) when high specificity is critical. Use this product when primer design has been optimized, but when an increase in specificity of the qPCR assay is still needed. TB Green Premix Ex Taq II (Tli RNase H Plus) includes TB Green, a reagent designed for intercalator-based qPCR. Additionally, TB Green Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly.The premixalso contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.

Our TB Green Master Mix productscontainTaKaRa Ex Taq DNA Polymerase Hot-Start Version, a hot-start PCR enzyme thatincludes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. TB Green Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performancewith GC-rich templates.