This reagent set is designed for simple and quick identification of type 1 and type 2 verocytotoxin genes from enterohemorrhagic E. coli. O-157:H7. The kit contains primer sets and positive controls for both genes, TaKaRa Ex Taq DNA Polymerase, dNTPs, and reaction buffer.
This reagent set is designed for simple and quick identification of type 1 and type 2 verocytotoxin genes from enterohemorrhagic E. coli. O-157:H7. The kit contains primer sets and positive controls for both genes, TaKaRa Ex Taq DNA Polymerase, dNTPs, and reaction buffer.
Control templates EC2 and EC3 are utilized as the positive control templates to verify PCR amplification, using each of the primer pairs for the detection of verocytotoxin genes from EHEC. Control template EC2 is designed to produce a 686-bp fragment with PCR primers EVT-1 and EVT-2. Control template EC3 is designed to produce a 686-bp fragment using PCR primers EVS-1 and EVS-2. Since the sizes of the amplified fragments from the control templates differ from the amplified fragments derived from verocytotoxin genes, target fragments are easily discriminated even if there is contamination in the control template tube.
