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当前位置: 首页 > 产品中心 > peptide > Clontech/Prelude一步前置放大器Master Mix有限RNA样本的高质量前置扩增/10 Rxns/638554
商品详细Clontech/Prelude一步前置放大器Master Mix有限RNA样本的高质量前置扩增/10 Rxns/638554
Clontech/Prelude一步前置放大器Master Mix有限RNA样本的高质量前置扩增/10 Rxns/638554
Clontech/Prelude一步前置放大器Master Mix有限RNA样本的高质量前置扩增/10 Rxns/638554
商品编号: 638554
品牌: Takara
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍

qPCR curves

Prelude One-Step PreAmp Master Mix uses an optimized reverse transcriptase, polymerase, and buffer to maximize RT-PCR efficiency for all targets in a preamplification reaction. This enables the highest level of unbiased preamplification for >100 targets, from as little as 100 pg mRNA or 10 ng total RNA. This 2X premix works with intact RNA or degraded RNA extracted from FFPE samples. Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target-enrichment analysis.

Prelude One-Step PreAmp Master Mix uses an optimized reverse transcriptase, polymerase, and buffer to maximize RT-PCR efficiency for all targets in a preamplification reaction. This enables the highest level of unbiased preamplification for >100 targets, from as little as 100 pg mRNA or 10 ng total RNA. This 2X premix works with intact RNA or degraded RNA extracted from FFPE samples. Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target-enrichment analysis.

qPCR, SNP genotyping, and targeted enrichment are powerful molecular techniques for a wide range of research and clinical applications. However, these techniques may be less effective when working with limited or precious starting materials, such as nucleic acids extracted from FFPE tissue, samples obtained in extremely small quantities, or material that occurs at dilute concentrations in biofluids.

These challenges can be overcome using preamplification (originally called booster PCR), which enables unbiased, target-specific amplification of cDNA and gDNA. The key consideration when performing preamplification is ensuring that your preamplification mix is sensitive enough to work with low-input amounts of template, while not introducing bias. Bias can occur when PCR efficiency varies for some of the targets and results in their under- or over-representation in the preamplified sample, which can then influence the data generated by downstream assays. Prelude One-Step PreAmp Master Mix allows unparalleled sensitivity and efficiency, ensuring the even amplification of many targets from very low RNA input amounts and giving you confidence in your results.

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品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。