Clontech/高效质粒DNA浓缩和脱盐，无需真空歧管核自旋终饰器Midi/50 Preps/740439.50-免疫在线蚂蚁淘旗下平台

商品详细Clontech/高效质粒DNA浓缩和脱盐，无需真空歧管核自旋终饰器Midi/50 Preps/740439.50

Clontech/高效质粒DNA浓缩和脱盐，无需真空歧管核自旋终饰器Midi/50 Preps/740439.50

商品编号： 740439.50

品牌： Takara

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：多肽合成

公司分类： peptide

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Eluates from anion-exchange plasmid preparations (such as NucleoBond Midi preps and Maxi preps) typically contain high salt concentrations. In addition to the vacuum-based NucleoSnap Finisher Midi/Maxi kits, Takara Bio offers NucleoSpin Finisher Midi, which enables rapid concentration and desalination of plasmid DNA using successive centrifugation steps.

Principle/procedure

NucleoSpin Finisher Midi kits supersede the commonly used and time-consuming isopropanol precipitation of DNA from anion exchange eluates by allowing the rapid DNA precipitation onto a filter membrane, followed by a combined washing/drying step and a convenient elution with a variable elution volume. All steps are performed in a centrifuge, which allows the parallel purification of multiple samples.

DNA is precipitated by Buffer FB and filtered by the specially designed matrix of the novel NucleoSpin Finisher Columns, followed by a combined washing and drying step with ethanolic Wash Buffer A4 to remove salts, impurities, and ethanol.

Afterwards, plasmid DNA can be eluted in supplied endotoxin-free H₂O-EF. The applied elution volume can be adjusted from 100μl to 1.5 ml, according to the expected amount of plasmid DNA, to ensure optimal yield and concentration.

Desalt and concentrate plasmid midipreps and maxipreps.

Application data

Ultra-fast plasmid DNA desalting and concentration.

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品牌介绍

50多年来，Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂，仪器和服务产品组合支持生命科学领域的进步，包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分，Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂（这是同类工厂中的第一个）能够对病毒，细胞和蛋白质进行无缝和无菌处理。我们的北美（NA）总部位于加利福尼亚山景城，我们在这里从事大部分研发，营销，质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究，物流和外展计划做出了贡献。此外，我们在瑞典哥德堡拥有GMP干细胞设施，在法国的Takara Bio Europe总部也为欧洲，中东和非洲的部分地区提供服务。