The NucleoVac 96 Vacuum Manifold is designed for the rapid parallel purification of nucleic acids using 8-well and 96-wellNucleoSpin kitsand theNucleoFast kits. The NucleoSpin 8/96 kits are based on silica-membrane technology and allow rapid simultaneous purification of high-quality nucleic acids. Time-consuming centrifugation and decanting steps are eliminated by using a vacuum.
The NucleoVac 96 Vacuum Manifold is designed for the rapid parallel purification of nucleic acids using 8-well and 96-well NucleoSpin kits and the NucleoFast kits. The NucleoSpin 8/96 kits are based on silica-membrane technology and allow rapid simultaneous purification of high-quality nucleic acids. Time-consuming centrifugation and decanting steps are eliminated by using a vacuum. The NucleoFast kits are based on ultrafiltration technology and offer a fast and convenient method for nucleic acid purification. The NucleoVac 96 manifold provides spacer sets to accommodate suitable microtiter plates, deep-well blocks (round-well and square-well blocks), or microtube racks for collecting samples.
- To use the NucleoSpin 8 kits on the NucleoVac 96, the Starter Kit A is needed. This set contains the Column Holders A and NucleoSpin Dummy Strips. The Column Holders A are used to hold the desired number of 8-well strips (up to six) during the preparation. The NucleoSpin Dummy Strips are used to fill the unused rows of the Column Holders A.
- If using a NucleoSpin 8 kit with filter and binding strips, please refer to the user manual for setup of the vacuum manifold. If the NucleoSpin 8 kit contains only binding strips, please see Figure 4.2 in the NucleoVac user manual.
- Using the NucleoSpin 96 kits with the NucleoVac 96 Vacuum Manifold requires no additional hardware. When using a kit with filter and binding plates (e.g., NucleoSpin 96 Plasmid), the setup is shown in Section 5.1 of the NucleoVac user manual. The setup for NucleoSpin 96 kits with only binding plates is shown in Section 5.2 of the NucleoVac user manual.
- Make sure to insert the appropriate spacers into the NucleoVac 96 Manifold Base, such that the binding strips/plate, the MN Wash Plate, the Rack of Tube Strips, or the Elution Plate is placed at the correct distance below the outlets of the plate inserted into the manifold top. When using the NucleoFast kits with the NucleoVac 96 Vacuum Manifold, insert a waste container into the manifold base. Close the manifold with the lid. Insert the NucleoFast Plate into the manifold lid and start the procedure according to the kit protocol. Unused wells do not need to be taped. It is not necessary to use the provided spacers. For the detailed setup instructions, refer to Section 6 of the NucleoVac user manual.
