NucleoSpin miRNA is suitable for the simultaneous isolation and purification of small RNA (<200 nt; e.g., miRNA, pre-miRNA, tRNA, 5S RNA), large RNA (>200 nt; e.g., mRNA, 18S rRNA, 28S rRNA, pre-miRNA), and protein in three separate fractions from a large variety of sample materials.
NucleoSpin miRNA is suitable for the simultaneous isolation and purification of small RNA (<200 nt; e.g., miRNA, pre-miRNA, tRNA, 5S RNA), large RNA (>200 nt; e.g., mRNA, 18S rRNA, 28S rRNA, pre-miRNA), and protein in three separate fractions from a large variety of sample materials.
The precipitated protein can easily be dissolved in Laemmli buffer and used for SDS-PAGE, Western Blot analysis, and protein quantification with the Macherey-Nagel Protein Quantification Assay. The eluted RNA and miRNA are ready-to-use for all standard downstream applications, such as RT-PCR, Northern blotting, and chip hybridization.
