The NucleoSpin totalRNA FFPE and NucleoSpin totalRNA FFPE XS kits provide a fast, efficient, and convenient method for isolating both small (e.g., miRNA) and large RNAs from formalin-fixed, paraffin-embedded (FFPE) tissue.

NucleoSpin totalRNA FFPE includes mini spin columns (30–50 µl elution volume) to purify standard FFPE samples (<50 mg tissue), while NucleoSpin totalRNA FFPE XS includes XS columns (5–30 µl elution volume) to purify very small FFPE samples (<5 mg tissue, e.g., microdissections). Both kits include blue-colored Paraffin Dissolver for easy visualization of phase separation and convenient paraffin removal without using toxic organic solvents (such as xylene), and rDNase for efficient on-column DNA removal. The kits combine decrosslinking and tissue lysis in one step, providing reliable yields of high-quality total RNA for enhanced RT-PCR performance. The NucleoSpin totalRNA FFPE XS kit makes it possible to isolate highly concentrated RNA suitable for qRT-PCR from even trace amounts of FFPE samples.