NucleoZOL is a highly efficient RNA-isolation reagent for extracting both small and large RNAs from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which, in turn, minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation.
NucleoZOL is a highly efficient RNA-isolation reagent for extracting both small and large RNAs from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which, in turn, minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation. NucleoZOL can be used to isolate total RNA in a single fraction, or miRNA and large RNA in two separate fractions. The NucleoZOL procedure can also be used in combination with NucleoSpin RNA columns.
The NucleoSpin RNA Set for NucleoZOL is a mini spin kit designed to combine NucleoZOL RNA extraction with NucleoSpin RNA purification. The kit contains silica membrane-based columns and the required buffers. After a sample is lysed in NucleoZOL (not provided in the mini spin kit), and contaminating DNA and proteins are pelleted, the supernatant is mixed with an optimized binding buffer for efficient binding of miRNA and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (miRNA and large RNA).
