The NucleoSpin RNA Plus kit is a next-generation RNA purification kit designed for quick, efficient RNAisolation from cells and tissues. This kit integrates a new feature, the NucleoSpin gDNA Removal Column, a mini spin column which quickly and efficiently removes genomic DNA contamination, thus eliminating the need for time-consuming rDNase digestion.

Cells and tissues are lysed in a lysis buffer that does not require the addition of beta-mercaptoethanol or TCEP since it contains a chaotropic salt that immediately inactivates any RNases. Thelysate is added to a NucleoSpin gDNA Removal Column for clarification and removal of contaminating gDNA. After addition of the binding solution to the flowthrough, the RNA is bound to a NucleoSpin RNA Plus Column. Two washes are performed to remove salts, metabolites, and macromolecular cellular components, and high-quality RNA is eluted with RNase-free water.

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