The NucleoSpin gDNA Clean-up and NucleoSpin gDNA Clean-up XS kits provide the easiest and fastest way to clean up and concentrate large genomic DNA fragments from enzymatic reactions or prepurified samples (extracted using organic solvents such as phenol/chloroform), or to perform buffer exchange.
The NucleoSpin gDNA Clean-up and NucleoSpin gDNA Clean-up XS kits provide the easiest and fastest way to clean up and concentrate large genomic DNA fragments from enzymatic reactions or prepurified samples (extracted using organic solvents such as phenol/chloroform), or to perform buffer exchange. These kits can be used to remove contaminants such as salts, enzymes, nucleotides, labeling reagents, etc., by selective binding to a silica membrane in a spin-column format using a simple bind-wash-elute procedure. The proven silica-membrane technology is an effective alternative to the widely used Centricon concentrator columns for cleaning and concentrating samples extracted by standard "organic" methods.
NucleoSpin gDNA Clean-up includes mini spin columns (50–100 µl elution volume) to purify standard samples (<150 µl aqueous DNA solution containing <25 µg DNA), while NucleoSpin gDNA Clean-up XS includes XS columns (6–10 µl elution volume) to purify very small samples (<400 µl aqueous DNA solution containing <2 µg DNA). The NucleoSpin gDNA Clean-up XS Column contains a unique silica membrane that allows the smallest elution volumes. Due to its high sensitivity, the XS kit is particularly well-suited for purifying trace levels of DNA from forensic samples. These kits reduce sample preparation time to 15–20 minutes, compared to >1 hour for ultrafiltration devices. The purified DNA is suitable for a variety of downstream applications, including PCR, enzymatic reactions, Southern blotting, qPCR, and STR amplification.