With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied columns, which are suitable for gel extraction as well as PCR purification, bind DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded onto the column, contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3—providing rapid DNA purification.
With the NucleoSpin Gel and PCR Clean-Up kits, you get two applications in one. The supplied columns, which are suitable for gel extraction as well as PCR purification, bind DNA to a silica membrane in the presence of a chaotropic salt. Once the binding mixture is loaded onto the column, contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3—providing rapid DNA purification. You can isolate fragments from 50 bp–20 kb in minimal elution volumes (as low as 15 µl for the mini kit, 200 µl for the midi kit, and 1 ml for the maxi kit) with recovery rates of 70–95%. Downstream applications include sequencing, ligation, labeling, cloning, and restriction enzyme digestion.
The NucleoSpin Gel and PCR Clean-Up kit was formerly known as the NucleoSpin Extract II kit.
