NucleoMag Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions.
NucleoMag Trace is designed for the purification of DNA from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters). After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation), the risk of cross-contamination is reduced. NucleoMag technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag SEP) or magnetic separators integrated into robotic workstations.
Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit typically yields 1–3 µg of genomic DNA in an elution volume of >50 µl at a concentration of 10–30 ng/µl. The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reactions.
