Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, and stroke. Circulating DNA is known to be highly fragmented and low in concentration, which makes it difficult to purify.
NucleoSpin cfDNA XS is designed to isolate fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit overcomes the limitations of conventional kits, namely large membrane diameters associated with large default elution volumes, and a resulting DNA concentration that is too low for direct use as a PCR template. NucleoSpin cfDNA XS allows elution in just 5–20 µl, resulting in highly concentrated DNA.
