The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The NucleoMag cfDNA kit is designed for rapid manual or automated isolation of circulating cell-free DNA (cfDNA) from 1–10 ml of human plasma obtained using EDTA or Cell-Free DNA BCT collection tubes. The kit enables efficient recovery of fragmented DNA ≥50 bp in size via reversible adsorption of nucleic acids to paramagnetic beads under optimized buffer conditions. cfDNA can be eluted in 50–200 µl of buffer and is ready for applications such as qPCR, ddPCR, NGS, etc.
The protocol provided in the NucleoMag cfDNA user manual is intended for processing 2-ml inputs of blood plasma, but kit reagents are provided in sufficient quantities for processing input volumes ranging from 1–10 ml. In comparison with a competitor kit developed for cfDNA purification from plasma, NucleoMag cfDNA was shown to provide higher, more consistent yields from challenging samples containing low amounts of cfDNA.
