NucleoSpin Blood, Blood L, Blood XL, and Blood QuickPure kits are designed for isolating genomic DNA from whole blood, serum, plasma, buffy coats, and cultured cells in a convenient silica membrane-based spin column format. Maximum starting volumes range from 200 µl of blood for the NucleoSpin Blood and Blood QuickPure mini kits to 2 ml for the Blood L midi kit and 10 ml for the Blood XL maxi kit. The NucleoSpin Blood QuickPure kits are ultra-fast mini kits that include spin columns with a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. This reduces the number of washing and drying steps fromthree to only one, resulting in a hands-on time of less than 10 minutes. DNA purified with any of these kits can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction.

NucleoSpin Blood mini kits only (not the Blood QuickPure & Blood L/XL kits) may also be used for purifying viral DNA for a wide variety of viruses from blood and biological fluids(including HBV, CMV, HPV, TTV, and EBV). The Proteinase K step included in the NucleoSpin Blood method removes viral proteins, allowing the viral DNA in the lysate to bind to the silica membrane. Since viral DNA copurifies with cellular DNA, we recommend using cell-free samples (serum or plasma) to prepare pure viral DNA. The purified DNA is suitable for typical downstream applications such as real-time PCR and enzymatic reactions.