NucleoMag Tissue is designed for manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions.
NucleoMag Tissue is designed for manual or automated small-scale preparation of genomic DNA from human and animal tissue, cultured cells, or bacteria, in a 96-well format. After lysis of the sample, genomic DNA is selectively bound to NucleoMag Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. Since the magnetic bead technology provides a closed system (no transfer of sample from one well to another during the preparation), the risk for cross contamination is reduced.
Typically, 96 samples can be purified in less than 120 minutes on common automation platforms. The kit provides reagents for the purification of 10–20 µg of genomic DNA from samples (e.g., 20 mg of tissue or 107 cells). The purified DNA is ready to use for PCR, Southern blotting, and any enzymatic reaction.
