NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates, and rapid lysate clearing, no centrifugation is required.
NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary. Lysates are cleared by simple filtration using the included special column filter, designed for high filter flow rates, and rapid lysate clearing, no centrifugation is required. Additionally NucleoBond Xtra has improved silica material with higher binding capacity, leading to typical yields of 400 μg (Midi) and 1000 μg (Maxi). NucleoBond Xtra Finalizers: The finalizers, provided with the Plus kits, replace the time-consuming centrifugation step that follows every isopropanol precipitation of diluted DNA samples.
