Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors (2i technology: CHIR99021 and PD0325901) that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.
Cellartis 2i mES/iPSC Culture Medium is a serum-free, defined cell culture medium for the establishment of mouse ES and iPS cell lines. This medium contains two selective small molecule inhibitors (2i technology: CHIR99021 and PD0325901) that act to eliminate differentiation-inducing signals from GSK3β and ERK/MEK, respectively, and promote cell proliferation.
The culture medium alone can be used for long-term maintenance of naive, ground state mouse embryonic stem cells or fully pluripotent mouse iPS cells. Addition of Leukemia Inhibitory Factor (LIF) can also induce the efficient conversion of partial- or pre-iPS cells into fully pluripotent iPS cells via Nanog (Silva et al. 2008; Silva et al. 2009).
The 2i technology has also been used to establish ES cells from mouse strains for which it is difficult to produce germline-competent ES cells (Nichols et al. 2009) and to induce iPS cells from human and livestock species (Wang et al. 2011; Malaver-Ortega et al. 2012).
