Ecotropic receptor booster technologies are used to temporarily elevate the density of viral receptor proteins on the surface of any target cell that you wish to infect. The technology allows you to efficiently transduce cells that are resistant to viral infection due to low receptor density. The Ecotropic Receptor Booster, for example, allows you to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.
Receptor booster technologies are used to temporarily elevate the density of viral receptor proteins on the surface of any target cell that you wish to infect. The technology allows you to efficiently transduce cells that are resistant to viral infection due to low receptor density. The Ecotropic Receptor Booster allows you to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.
How does it work?
Each receptor booster consists of a vial of concentrated exosome-like vesicles that are coated with the mCAT-1 receptor protein. When the booster vesicles are applied to your cells (10–20µl per well of a 6-well plate for ~2hours), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic retrovirus.
We recommend the following products for producing ecotropic or amphotropic virus:
Ecotropic lentivirus:Lenti-X Packaging Single Shots (Ecotropic)
- Ecotropic retrovirus:Retro-X Universal Packaging System
