The SMARTer Human TCR a/b Profiling Kit v2 (TCRv2) is powered by robust chemistry that provides unparalleled sensitivity and reproducibility. The kit leverages SMART (Switching Mechanism at 5" end of RNA Template) full-length cDNA synthesis technology and pairs NGS with a 5"-RACE approach to capture the complete V(D)J variable regions of TRA and TRB genes.

The kit is designed to work with a range of RNA input amounts (RIN ≥8; depending on the sample type) and has been shown to yield high-quality sequencing libraries from as little as 10 ng to 1 µg of total RNA obtained from peripheral blood leukocytes, 1 ng to 100 ng of total RNA obtained from T cells, or from 1,000 to 10,000 purified, whole T cells. Libraries can be generated to obtain both alpha and beta chain diversity information. This latest profiling kit also includes unique molecular identifiers (UMI), making it possible to remove reads derived from PCR duplicates and sequencing errors, thus ensuring more accurate and reliable results. In addition to the reagents, the TCRv2 kit includes a dual index (UDI) plate to generate Illumina-compatible sequencing libraries.

Once output files are generated with an Illumina sequencer, they can be analyzed using the Cogent NGS Immune Profiler Software. This software enables high-quality TCR profiling analysis, including count and identification of clonotypes and sequence information for V(D)J regions.

Benefits of this kit include:

• Use of UMIs to correct for PCR and sequencing errors
• Addition of UDIs, allowing for greater confidence in sequencing on a patterned flow cell (such as the NovaSeq™ system) and the ability to pool a greater number of samples
• Flexibility to sequence on any Illumina instrument
• Full-length V(D)J analysis on the MiSeq™ system
• Access to Cogent NGS Immune Profiler Software, an easy-to-use analysis pipeline toolfor users of any bioinformatic experience level, for faster data analysis