The Ready-To-Glow Secreted Luciferase Reporter Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based assay.
Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using western blot analysis.
The Ready-To-Glow Secreted Luciferase Reporter Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system.
Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using western blot analysis.
Benefits of using a live-cell assay
| Ready-To-Glow Secreted Luciferase Reporter Assay | Firefly and Renilla-Based Reporter Assays | |
| One-step protocol, without cell lysis | Yes. Reporter molecule is naturally secreted into the media and can be assayed without lysing cells. | No. |
| 2–4 fold higher signal than Renilla or firefly assays | Yes. | No. |
| Multiple data points per well | Yes. Single well can be used for multiple time points, as there is no cell lysis. | No. |
| Multiple compounds/trigger testing on same cells (single transfection) | Yes. | No. |
| Removal of background signal | Yes. Background signal can be reset to zero by removing and replacing the media | No. |
Available format
The Ready-To-Glow Secreted Luciferase Reporter Assay includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
Dual secreted reporter assay—luciferase & SEAP
The Ready-To-Glow Dual Secreted Reporter Assay has two live-cell reporters that do not require cell lysis. In addition to thesecreted luciferase assay,it includes a secretedversion ofalkaline phosphatase.
