The Random Primer DNA Labeling Kit Ver. 2 is designedfor radioactivelabeling for hybridization probes. Probe labeling can label DNA with [32P]-alpha-, [35S]-alpha-, or [3H]-alpha-dCTP. This random primer DNA labelingkit is based on a modified methodby Feinberg and Vogelstein (Feinberg and Vogelstein 1983; Feinberg and Vogelstein 1984), and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I,Klenow fragment.Usingprimers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This probelabelingmethod overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 ng to 20 ng).
