The Advantage GC 2 Polymerase Mix is specially formulated to amplify GC-rich templates. It includes the high-yield and high-fidelity Advantage 2 enzyme blend plus a proprietary GC-Melt Reagent and a reaction buffer containing DMSO—two additives proven to enable the amplification of templates with up to 90% GC content with minimal buffer optimization. The combination of DMSO and GC-Melt Reagent allow the amplification of virtually all GC-rich sequences that are resistant to PCR amplification by standard techniques.

For a complete kit, use the Advantage GC 2 PCR Kit (Cat. # 639120, 639119) which includes the enzyme blend, GC buffers, and dNTPs, plus GC-rich control template and primers.

The Advantage GC 2 polymerase mix and PCR kit are ideal for use with cDNA templates. For amplifying regions of GC-rich mammalian genomic DNA, we recommend using Advantage GC Genomic LA Polymerase Mix.

If you are interested in Commercial Use of our products, including OEM, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Business Development at bd_oem@takarabio.com to discuss your needs, or visit our OEM page to submit an inquiry.