The SMARTer Mouse BCR IgG H/K/L Profiling Kit pairs 5" RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling. The 5"-RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.
The SMARTer Mouse BCR IgG H/K/L Profiling Kit pairs 5" RACE with NGS technology to provide a sensitive, accurate, and optimized approach to BCR profiling. The 5"-RACE method reduces variability and allows for priming from the constant region of BCR heavy or light chains. This kit combines these benefits with gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts and provide a highly sensitive and reproducible method for profiling B-cell repertoires.
The kit is designed to work with a range of RNA input amounts depending on the sample type, and has been shown to generate high-quality, Illumina-ready libraries from as little as 10 ng to 3 µg of total RNA from spleen, lymph node, peripheral blood mononuclear cells (PBMCs), and hybridomas.
Downstream sequencing of H/K/L chains allows for accurate identification of top clonotypes and reliable assignment of isotype in a majority of cases.
