Obtain full-length sequences of TCR-alpha and TCR-beta V(D)J variable regions
NOTE:
For TCR sequencing, we recommend the upgraded version of the kits below, the SMARTer Human TCR a/b Profiling Kit v2 (TCR v2). Based on the same great chemistry, TCR v2 improves upon it with UMIs for confident clonotype calls, UDIs for increased multiplexing capabilities, and easy-to-use bioinformatics software for an end-to-end solution.
Cat. # 635014 and 635015 will be discontinued by early 2021.
Obtain full-length sequences of TCR-alpha and TCR-beta V(D)J variable regions
NOTE:
For TCR sequencing, we recommend the upgraded version of the kits below, the SMARTer Human TCR a/b Profiling Kit v2 (TCR v2). Based on the same great chemistry, TCR v2 improves upon it with UMIs for confident clonotype calls, UDIs for increased multiplexing capabilities, and easy-to-use bioinformatics software for an end-to-end solution.
Cat. # 635014 and 635015 will be discontinued by early 2021.
The SMARTer Human TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor (TCR) repertoire analysis using NGS. The kit employs a 5’ RACE-like approach to capture complete V(D)J variable regions of TCR transcripts, starting from human blood RNA samples (10 ng–3 µg) or directly from intact lymphocytes (50–10,000 purified T cells). As the name suggests, the kit can be used to generate data for both TCR-alpha and TCR-beta chain diversity, either in the same experiment or separately. In contrast with TCR profiling methods that involve PCR multiplexing, each round of library amplification utilizes a single primer pair for each TCR subunit. Libraries produced with the kit are indexed and ready for sequencing on Illumina platforms.
This kit leverages our proven SMART technology (Switching Mechanism at 5’ End of RNA Template) along with LNA technology to amplify TCR mRNA sequences in an unbiased manner with remarkable sensitivity.
