Western BLoT Rapid Detect v2.0 is a detection reagent kit for Western blotting that detects primary antibodies using an antibody-free IgG detector solution (HRP-labeled). Compared with conventional detection techniques that use labeled secondary antibodies, this product provides faster, higher sensitivity detection and can be used for simultaneous detection of multiple types of primary antibodies. An added blocking component in the v2 dilution buffer allows for even higher sensitivity and simpler use.
The main component of this product is the IgG detector, an HRP conjugate that binds IgG. On the surface of one molecule of IgG Detector there are approximately 100 antibody-binding protein molecules and approximately 50 molecules of HRP.
The performance of this kit has been validated in combination with the Western BLot HRP chemiluminescent substrate series.
