These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.
These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.
Dropout supplements
Dropout (DO) supplements provide various mixtures of amino acids and nucleotides to add to the Minimal SD Bases to make a defined minimal medium. In each DO mix, certain amino acids have been “dropped out”, for example the -Leu/-Trp DO supplement contains every essential amino acid except for leucine and tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli. Each package will make at least 10 L of medium.
