These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for usingG418.
These vectors are ideally suited as controls to measure transfection efficiency. Simply co-transfect a red fluorescent protein vector with the target plasmid, and the resulting cells that are labeled with the fluorescent protein will also likely be transfected with the experimental plasmid. Each vector expresses a fluorescent protein from a constitutive promoter (CMV or EF1-alpha) and, if required, stable transfected cells can be selected for using G418. Transfected cells can also be used in assays that require fluorescent labeling of whole cells.
These vectors do not contain a multiple cloning site; other fluorescent protein vectors are available for cloning applications. Click here for green fluorescent protein options.
