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当前位置: 首页 > 产品中心 > Tag_antibody > 克隆技术/3G诱导表达系统上的逆转录病毒Tet/Each/631188
商品详细克隆技术/3G诱导表达系统上的逆转录病毒Tet/Each/631188
克隆技术/3G诱导表达系统上的逆转录病毒Tet/Each/631188
克隆技术/3G诱导表达系统上的逆转录病毒Tet/Each/631188
商品编号: 631188
品牌: Takara
市场价: ¥730000.00
美元价: 438000.00
产地: 美国(厂家直采)
公司:
产品分类: 标签抗体
公司分类: Tag_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Combines the efficiency of retroviral gene transfer with the power of our premium Tet-On 3G gene expression technology

This page describes a two-vector retrovirus format for inducible expression.Click this link to learn more about our all-in-one Retro-X Tet-One inducible expression systems.

The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology.

This page describes a two-vector retrovirus format for inducible expression. Click this link to learn more about our all-in-one Retro-X Tet-One inducible expression systems.

The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology. The retroviral vectors in this system can be used to establish a tightly controlled inducible gene expression system in a wide variety of dividing hard-to-transfect cell types.

How it works

Doxycycline-controlled transactivators are fusion proteins that contain a DNA-binding TetR domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G, Tet-On Advanced and Tet-Off Advanced proteins). With very high specificity, these transactivators recognize and bind to a tetracycline response element (TRE) that is located in the PTRE3GV promoter upstream of your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. The Tet-On 3G system is the premium, third-generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.

In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium.

Ideal retroviral packaging

Retro-X Tet-On 3G systems are supplied with our Retro-X Universal Packaging System (Cat. No. 631530) which produces high titers of MMLV retrovirus and allows you to customize the tropism of your retrovirus to efficiently transduce any cell line or primary cell culture, and thus greatly expands the applications for these inducible systems.

Retro-X Q vector technology

The vectors in these systems feature Takara Bio"s Retro-X Q-Series technology, which:

  • Drives high-titer virus production in packaging cells
  • Inactivates viral LTRs in target cells to prevent promoter interference
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品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。