The RiboGone - Mammalian kit specifically removes ribosomal RNA (rRNA) and mitochondrial RNA (mtRNA) from human, mouse, or rat total RNA samples. RiboGone - Mammalian uses oligo hybridization and RNase H digestion to specifically deplete 5S, 5.8S, 18S, and 28S nuclear rRNA, as well as 12S mtRNA. The kit is designed to work with input RNA of any quality, including degraded material (e.g., FFPE samples). RiboGone - Mammalian is highly suited for sample preparation prior to random-primed cDNA synthesis and RNA-seq or transcriptome analysis applications. When used in tandem with the SMARTer Stranded RNA-Seq Kit or the SMARTer Universal Low Input RNA Library Prep Kit, RiboGone - Mammalian reduces the frequency of rRNA sequences to <5% of total reads.
