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当前位置: 首页 > 产品中心 > Isotype_control_antibody > Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
商品详细Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
Clontech/Differential expression analysis on the ICELL8 Single-Cell System/1 Rxn/640143
商品编号: 640143
品牌: Takara
市场价: ¥590000.00
美元价: 354000.00
产地: 美国(厂家直采)
公司:
产品分类: 同型对照抗体
公司分类: Isotype_control_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

NGS 3' DE workflow

By leveraging Takara Bio"s expertise in single-cell RNA-seq library construction and the cell isolation and selection capabilities of the ICELL8 platform, the ICELL8 3" DE and ICELL8 3" DE for UMI workflows enable high-throughput preparation of Illumina libraries for differential expression analysis of single cells from up to eight different samples in parallel.

By leveraging Takara Bio"s expertise in single-cell RNA-seq library construction and the cell isolation and selection capabilities of ICELL8 systems, the ICELL8 3" DE and ICELL8 3" DE for UMI workflows enable high-throughput preparation of Illumina libraries for differential expression analysis of single cells from up to eight different samples in parallel.

The ICELL8 Chip and Reagent 3" DE Kit (Cat. # 640164, 640165, 640166) is compatible with the original ICELL8 Single-Cell System. Included in the kit are an ICELL8 3" DE Chip (Cat. # 640143), a 5,184-nanowell SmartChip that contains preprinted oligo(dT) primers, and reagents for cDNA synthesis and Illumina library prep.

Following staining and dilution, sample cells are dispensed into chip nanowells using the ICELL8 MultiSample NanoDispenser (MSND). Wells containing single, viable cells are selectedusing the ICELL8 Imaging System and the CellSelect Software provided with the ICELL8 system. Following the selection of cells for processing and analysis, RT-PCR reagents are dispensed into the corresponding wells, and cDNA synthesis and amplification are performed in-chip in a single run. Following RT-PCR, cDNA products are pooled and purified, and sequencing libraries are prepared using a transposase-based method that enriches for cDNA derived from mRNA 3" ends, followed by a limited-cycle PCR amplification step that incorporates Illumina index sequences.

Sequencing data from the resulting libraries can be traced back to individual cells using the nanowell-specific barcode sequences and can be used to perform differential gene expression analysis by mRNA 3" end-counting. By yielding high-quality, 3"-focused sequencing data from hundreds of cells in parallel, these kits provide an efficient, cost-effective solution for high-throughput differential expression analysis of single cells.

The ICELL8 3" DE for UMI Reagent Kit is used with the ICELL8 3" DE Chip as part of the ICELL8 3" DE with UMI workflow, which enables Illumina sequencing and differential gene expression analysis-via transcript 3" end counting-of single cells isolated on the ICELL8 system.

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品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。