4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > PCR_instrument > Clontech/T-Vectors pMD20 and pMD19 for PCR cloning/1 ug/3271
商品详细Clontech/T-Vectors pMD20 and pMD19 for PCR cloning/1 ug/3271
Clontech/T-Vectors pMD20 and pMD19 for PCR cloning/1 ug/3271
Clontech/T-Vectors pMD20 and pMD19 for PCR cloning/1 ug/3271
商品编号: 3271
品牌: Takara
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: PCR仪
公司分类: PCR_instrument
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

pMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (cleaved by EcoRV) with single 3’-terminal thymidine residues (dT) at both ends. These T-overhangs at the cloning site improve the efficiency of ligation of PCR products that contain dA-overhangs. The inclusion of lacZ in these vectors allows blue/white screening for the detection of successful ligations.

pMD20 and pMD19 (Simple) T-Vectors are linearized PCR cloning vectors (cleaved by EcoRV) with single 3"-terminal thymidine residues (dT) at both ends. These T-overhangs at the cloning site improve the efficiency of ligation of PCR products that contain dA-overhangs. The inclusion of lacZ in these vectors allows blue/white screening for the detection of successful ligations.

T-Vector pMD19 (Simple) is derived from pUC19 and has deletions of all of the restriction enzyme sites in the multiple-cloning site (MCS). Therefore, after cloning, restriction enzyme digestion analysis of the PCR insert is possible. Alternatively, T-Vector pMD20 retains the MCS of pUC19. In addition, this vector contains the SP6 promoter upstream of MCS. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase.

Both vectors are supplied with Control Insert DNA (500 bp) for use as a positive control.

 More Less
品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。