Uni-Link AminoModifier is a cyanoethyl phosphoramidite that directly incorporates primary aliphatic amines into oligonucleotides, providing an efficient way to directly label oligos with various reporter molecules (Nelson, Kent, and Muthini 1992; Nelson and Muthini 1993; Nelson et al. 1993; Nelson and Muthini 1993).Uni-Link AminoModifier possesses the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:
Uni-Link AminoModifier is a cyanoethyl phosphoramidite that directly incorporates primary aliphatic amines into oligonucleotides, providing an efficient way to directly label oligos with various reporter molecules (Nelson, Kent, and Muthini 1992; Nelson and Muthini 1993; Nelson et al. 1993; Nelson and Muthini 1993).Uni-Link AminoModifier possesses the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offers the following distinct labeling advantages:
- Multiple labels can be incorporated because phosphoramidites do not truncate chain elongation at 5" termini.
- Natural 3-carbon internucleotide phosphodiester distance is maintained, providing better hybridization and annealing properties than those obtained with other labeling methods.
- Attached label is separated from the phosphodiester connection point to reduce steric hindrance. The ABPD backbone has a 7-atom spacer arm that separates the label from the connection point.
- Label incorporation can be easily measured by standard trityl cation assays because phosphoramidites possess a dimethoxytrityl (DMT)-protected hydroxyl group.
Please inquire about bulk orders or custom synthesis requests at Technical_Support@takarabio.com.
