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当前位置: 首页 > 产品中心 > Gene_library > 克隆技术/用于SNV和CNV检测的单细胞文库制备/24 Rxns/R300669
商品详细克隆技术/用于SNV和CNV检测的单细胞文库制备/24 Rxns/R300669
克隆技术/用于SNV和CNV检测的单细胞文库制备/24 Rxns/R300669
克隆技术/用于SNV和CNV检测的单细胞文库制备/24 Rxns/R300669
商品编号: R300669
品牌: Takara
市场价: ¥600000.00
美元价: 360000.00
产地: 美国(厂家直采)
公司:
产品分类: 文库
公司分类: Gene_library
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

PicoPLEX Gold DNA-Seq

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection.

The PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured from the original versions of PicoPLEX kits and does not include indexes. Compatible index kits are described below and can be purchased here.

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection.

The PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured from the original versions of PicoPLEX kits and does not include indexes. Compatible index kits are described below and can be purchased here.

PicoPLEX WGA (PicoPLEX) quasi-random priming technology is the international gold standard for whole genome amplification (WGA) for subsequent detection of CNVs in fixed or unfixed single cells. Initially, PicoPLEX chemistry was created for the reproducible detection of aneuploidies and CNVs. To address the need for accurate detection of SNVs, we further optimized the PicoPLEX WGA chemistry using enzymes, primers, and protocols that improve sequencing coverage, uniformity, and accuracy while increasing the resolution for CNVs, SNVs, indels, and other small structural variants while retaining reproducibility.

The enhanced chemistry named PicoPLEX Gold is a single-cell library prep kit with a simple, four-step protocol to convert fixed or unfixed single cells into NGS libraries in under three hours with minimum hands-on time.

DNA HT Dual Index kits

DNA HT Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660–R400663); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.

DNA Unique Dual Index kits

DNA Unique Dual Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.

DNA Single Index kits

DNA Single Index kits are designed for use with ThruPLEX and PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.

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品牌介绍
50多年来,Takara Bio一直致力于通过我们的创新技术帮助科学家开拓新的领域。我们广泛的试剂,仪器和服务产品组合支持生命科学领域的进步,包括一线诊断和临床应用。我们是Takara Bio Inc.的一部分,Takara Bio Inc.是总部位于日本滋贺市的生物技术研究与开发的全球领导者。我们在日本西部的GMP制造工厂(这是同类工厂中的第一个)能够对病毒,细胞和蛋白质进行无缝和无菌处理。我们的北美(NA)总部位于加利福尼亚山景城,我们在这里从事大部分研发,营销,质量保证和电子商务。我们在密歇根州安阿伯市和威斯康星州麦迪逊市的其他NA地点为我们的研究,物流和外展计划做出了贡献。此外,我们在瑞典哥德堡拥有GMP干细胞设施,在法国的Takara Bio Europe总部也为欧洲,中东和非洲的部分地区提供服务。